Welcome to my blog, I'm Charles Johnson - a scientist and an amateur photographer. This site is devoted to all aspects of science related to photography. For more information read my About page.

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Charles S. Johnson, Jr.
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Comments[0] 05:14PM Jul 15, 2007 by Charles in General
Here Prof. Hart compares various programs and concludes that the freeware program CombineZX does rather well. Information about Combine ZM can be found at:
http://www.hadleyweb.pwp.blueyonder.co.uk/CZM/Manual/combinezm_help.htm
A couple of weeks ago I downloaded CombineZN and finally got
around to testing it. I have only used
the automatic mode, but I am impressed.
Here I show a few images to serve as an illustration. The shots were taken with a Sigma 150mm lens
at close to 1:1 focus (1X magnification) with 68mm of extension tubes added to
bring the magnification to 1.68. In all
cases I used f/5.6. I took seven shots
with a change in focus the same direction each time. These are photos of a fly about 2mm in length
that has been trapped by a
By the way, the plant is small. This photo (228 kb, jpg) puts it all into context.
Comments[0] 04:11PM Jul 15, 2007 by Charles in General
Avoiding diffraction problems in macro-photography: We all know about the loss of resolution that
occurs with small apertures (large f#’s), but it is easy to forget that the
effective f# is not equal to the one shown in the camera. The f# we need to use is: f#(effective) = f# times (1 + m). Here m is the magnification. My Sigma 150mm macro lens will focus to 1X
magnification. At that point all the
f#’s shown should be multiplified by two in order to estimate the DoF and the
effect of diffraction. It gets worse
with extension tubes. I add 68mm of
additional extension to get 1.68X and the f#
shown needs to be multiplied by 2.68!
When I forgot this factor and set the camera for f# = 22, I got
unsatisfactory images. As an example
take a look at this figure:
Sundew plant (100% crop of 1X macro photos) (635 kb, jpg)